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Selective Plane Illumination Microscopy (SPIM), also widely known as Light Sheet Fluorescence Microscopy (LSFM), is a revolutionary 3D imaging technique that has redefined how scientists study living biological systems. Unlike traditional microscopes that illuminate an entire sample at once, SPIM uses a micron-thin sheet of laser light to illuminate only a single optical section at a time.

By separating the illumination path from the detection path, SPIM captures dynamic cellular environments with unprecedented speed and minimal damage. ⚙️ How SPIM Works: The Orthogonal Design

The defining technical feature of SPIM is its 90-degree orthogonal architecture:

Selective plane illumination microscopy techniques in developmental biology – PMC